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Since they are devoid of any cell wall, the fusion of ) /K [9] /P 93 0 R /Pg 6 0 R >> endobj 96 0 obj << /S /Span /Type /StructElem /ActualText (protoplasts becomes easy without facing any incompatibility barriers, while fusing the two genomes. ) /K [10] /P 93 0 R /Pg 6 0 R >> endobj 93 0 obj << /S /LBody /Type /StructElem /K [92 0 R 95 0 R 96 0 R] /P 94 0 R >> endobj 94 0 obj << /S /LI /Type /StructElem /K [93 0 R] /P 80 0 R >> endobj 80 0 obj << /S /L /Type /StructElem /K [79 0 R 85 0 R 90 0 R 94 0 R] /P 81 0 R >> endobj 81 0 obj << /S /Textbox /Type /StructElem /K [80 0 R] /P 76 0 R >> endobj 97 0 obj << /S /Figure /Type /StructElem /K [11] /P 76 0 R /Pg 6 0 R >> endobj 98 0 obj << /S /Figure /Type /StructElem /K [12] /P 76 0 R /Pg 6 0 R >> endobj 76 0 obj << /S /Slide /Type /StructElem /K [75 0 R 81 0 R 97 0 R 98 0 R] /P 3 0 R >> endobj 103 0 obj << /S /Span /Type /StructElem /K [0] /P 104 0 R /Pg 7 0 R >> endobj 105 0 obj << /S /Span /Type /StructElem /ActualText /K [1] /P 106 0 R /Pg 7 0 R >> endobj 110 0 obj << /S /Span /Type /StructElem /ActualText (1. Fusion of Protoplast) /K [2] /P 106 0 R /Pg 7 0 R >> endobj 111 0 obj << /S /Span /Type /StructElem /K [3] /P 106 0 R /Pg 7 0 R >> endobj 106 0 obj << /S /LBody /Type /StructElem /K [105 0 R 110 0 R 111 0 R] /P 107 0 R >> endobj 107 0 obj << /S /LI /Type /StructElem /K [106 0 R] /P 108 0 R >> endobj 112 0 obj << /S /Span /Type /StructElem /ActualText /K [4] /P 113 0 R /Pg 7 0 R >> endobj 115 0 obj << /S /Span /Type /StructElem /ActualText (The fusion of protoplasts can be achieved by three methods:) /K [5] /P 113 0 R /Pg 7 0 R >> endobj 113 0 obj << /S /LBody /Type /StructElem /K [112 0 R 115 0 R] /P 114 0 R >> endobj 114 0 obj << /S /LI /Type /StructElem /K [113 0 R] /P 108 0 R >> endobj 116 0 obj << /S /Span /Type /StructElem /ActualText /K [6] /P 117 0 R /Pg 7 0 R >> endobj 119 0 obj << /S /Span /Type /StructElem /ActualText (a\) Mechanical fusion: ) /K [7] /P 117 0 R /Pg 7 0 R >> endobj 120 0 obj << /S /Span /Type /StructElem /ActualText (The protoplasts are allowed to fuse mechanically by putting them ) /K [8] /P 117 0 R /Pg 7 0 R >> endobj 121 0 obj << /S /Span /Type /StructElem /ActualText (together on a depression slide. However, this technique can often destroy the protoplasts.) /K [9] /P 117 0 R /Pg 7 0 R >> endobj 117 0 obj << /S /LBody /Type /StructElem /K [116 0 R 119 0 R 120 0 R 121 0 R] /P 118 0 R >> endobj 118 0 obj << /S /LI /Type /StructElem /K [117 0 R] /P 108 0 R >> endobj 122 0 obj << /S /Span /Type /StructElem /ActualText /K [10] /P 123 0 R /Pg 7 0 R >> endobj 125 0 obj << /S /Span /Type /StructElem /ActualText (b\) Spontaneous fusion: ) /K [11] /P 123 0 R /Pg 7 0 R >> endobj 126 0 obj << /S /Span /Type /StructElem /ActualText (This is a natural process that happens during the enzymatic ) /K [12] /P 123 0 R /Pg 7 0 R >> endobj 127 0 obj << /S /Span /Type /StructElem /ActualText (degradation of cell walls. The surrounding protoplasts often fuse together, without any ) /K [13] /P 123 0 R /Pg 7 0 R >> endobj 128 0 obj << /S /Span /Type /StructElem /ActualText (physical interference, but these fusions cannot give rise to whole plants.) /K [14] /P 123 0 R /Pg 7 0 R >> endobj 123 0 obj << /S /LBody /Type /StructElem /K [122 0 R 125 0 R 126 0 R 127 0 R 128 0 R] /P 124 0 R >> endobj 124 0 obj << /S /LI /Type /StructElem /K [123 0 R] /P 108 0 R >> endobj 129 0 obj << /S /Span /Type /StructElem /ActualText /K [15] /P 130 0 R /Pg 7 0 R >> endobj 132 0 obj << /S /Span /Type /StructElem /ActualText (c\) Induced fusion: ) /K [16] /P 130 0 R /Pg 7 0 R >> endobj 133 0 obj << /S /Span /Type /StructElem /ActualText (Isolated protoplasts are fused together with the help of chemicals called ) /K [17] /P 130 0 R /Pg 7 0 R >> endobj 134 0 obj << /S /Span /Type /StructElem /ActualText (fusogens) /K [18] /P 130 0 R /Pg 7 0 R >> endobj 135 0 obj << /S /Span /Type /StructElem /ActualText (, such as NaNO3, PEG, polyvinyl alcohol, lysozyme, dextran, fatty acids, ) /K [19] /P 130 0 R /Pg 7 0 R >> endobj 136 0 obj << /S /Span /Type /StructElem /ActualText (electrofusion, etc. ) /K [20] /P 130 0 R /Pg 7 0 R >> endobj 130 0 obj << /S /LBody /Type /StructElem /K [129 0 R 132 0 R 133 0 R 134 0 R 135 0 R 136 0 R] /P 131 0 R >> endobj 131 0 obj << /S /LI /Type /StructElem /K [130 0 R] /P 108 0 R >> endobj 137 0 obj << /S /Span /Type /StructElem /ActualText /K [21] /P 138 0 R /Pg 7 0 R >> endobj 140 0 obj << /S /Span /Type /StructElem /ActualText (The mechanism of induced fusion is described below:) /K [22] /P 138 0 R /Pg 7 0 R >> endobj 138 0 obj << /S /LBody /Type /StructElem /K [137 0 R 140 0 R] /P 139 0 R >> endobj 139 0 obj << /S /LI /Type /StructElem /K [138 0 R] /P 108 0 R >> endobj 141 0 obj << /S /Span /Type /StructElem /ActualText /K [23] /P 142 0 R /Pg 7 0 R >> endobj 144 0 obj << /S /Span /Type /StructElem /ActualText (1) /K [24] /P 142 0 R /Pg 7 0 R >> endobj 145 0 obj << /S /Span /Type /StructElem /K [25] /P 142 0 R /Pg 7 0 R >> endobj 146 0 obj << /S /Span /Type /StructElem /ActualText (\) Agglutination/adhesion: ) /K [26] /P 142 0 R /Pg 7 0 R >> endobj 147 0 obj << /S /Span /Type /StructElem /ActualText (The two protoplasts adhere together when brought in close ) /K [27] /P 142 0 R /Pg 7 0 R >> endobj 148 0 obj << /S /Span /Type /StructElem /ActualText (contact by ) /K [28] /P 142 0 R /Pg 7 0 R >> endobj 149 0 obj << /S /Span /Type /StructElem /ActualText (fusogens) /K [29] /P 142 0 R /Pg 7 0 R >> endobj 150 0 obj << /S /Span /Type /StructElem /ActualText (, such as polyethylene glycol\(PEG\) and NaNO3.) /K [30] /P 142 0 R /Pg 7 0 R >> endobj 142 0 obj << /S /LBody /Type /StructElem /K [141 0 R 144 0 R 145 0 R 146 0 R 147 0 R 148 0 R 149 0 R 150 0 R] /P 143 0 R >> endobj 143 0 obj << /S /LI /Type /StructElem /K [142 0 R] /P 108 0 R >> endobj 151 0 obj << /S /Span /Type /StructElem /ActualText /K [31] /P 152 0 R /Pg 7 0 R >> endobj 154 0 obj << /S /Span /Type /StructElem /ActualText (2) /K [32] /P 152 0 R /Pg 7 0 R >> endobj 155 0 obj << /S /Span /Type /StructElem /K [33] /P 152 0 R /Pg 7 0 R >> endobj 156 0 obj << /S /Span /Type /StructElem /ActualText (\) Plasma membrane fusion: ) /K [34] /P 152 0 R /Pg 7 0 R >> endobj 157 0 obj << /S /Span /Type /StructElem /ActualText (The protoplasts membrane fuse together at the site of ) /K [35] /P 152 0 R /Pg 7 0 R >> endobj 158 0 obj << /S /Span /Type /StructElem /ActualText (adhesion, which forms a cytoplasmic bridge between the two protoplasts. High pH and ) /K [36] /P 152 0 R /Pg 7 0 R >> endobj 159 0 obj << /S /Span /Type /StructElem /ActualText (Ca2+ concentration can increase the rate of membrane fusion.) /K [37] /P 152 0 R /Pg 7 0 R >> endobj 152 0 obj << /S /LBody /Type /StructElem /K [151 0 R 154 0 R 155 0 R 156 0 R 157 0 R 158 0 R 159 0 R] /P 153 0 R >> endobj 153 0 obj << /S /LI /Type /StructElem /K [152 0 R] /P 108 0 R >> endobj 160 0 obj << /S /Span /Type /StructElem /ActualText /K [38] /P 161 0 R /Pg 7 0 R >> endobj 163 0 obj << /S /Span /Type /StructElem /ActualText (3) /K [39] /P 161 0 R /Pg 7 0 R >> endobj 164 0 obj << /S /Span /Type /StructElem /K [40] /P 161 0 R /Pg 7 0 R >> endobj 165 0 obj << /S /Span /Type /StructElem /ActualText (\) Formation of heterokaryons: ) /K [41] /P 161 0 R /Pg 7 0 R >> endobj 166 0 obj << /S /Span /Type /StructElem /ActualText (The fused protoplasts round up to form a spherical ) /K [42] /P 161 0 R /Pg 7 0 R >> endobj 167 0 obj << /S /Span /Type /StructElem /ActualText (homokaryon) /K [43] /P 161 0 R /Pg 7 0 R >> endobj 168 0 obj << /S /Span /Type /StructElem /ActualText (or heterokaryon.) /K [44] /P 161 0 R /Pg 7 0 R >> endobj 161 0 obj << /S /LBody /Type /StructElem /K [160 0 R 163 0 R 164 0 R 165 0 R 166 0 R 167 0 R 168 0 R] /P 162 0 R >> endobj 162 0 obj << /S /LI /Type /StructElem /K [161 0 R] /P 108 0 R >> endobj 108 0 obj << /S /L /Type /StructElem /K [107 0 R 114 0 R 118 0 R 124 0 R 131 0 R 139 0 R 143 0 R 153 0 R 162 0 R] /P 109 0 R >> endobj 109 0 obj << /S /Textbox /Type /StructElem /K [108 0 R] /P 104 0 R >> endobj 104 0 obj << /S /Slide /Type /StructElem /K [103 0 R 109 0 R] /P 3 0 R >> endobj 170 0 obj << /S /Span /Type /StructElem /K [0] /P 171 0 R /Pg 8 0 R >> endobj 172 0 obj << /S /Span /Type /StructElem /ActualText /K [1] /P 173 0 R /Pg 8 0 R >> endobj 177 0 obj << /S /Span /Type /StructElem /ActualText (1. Fusion of Protoplast) /K [2] /P 173 0 R /Pg 8 0 R >> endobj 178 0 obj << /S /Span /Type /StructElem /K [3] /P 173 0 R /Pg 8 0 R >> endobj 173 0 obj << /S /LBody /Type /StructElem /K [172 0 R 177 0 R 178 0 R] /P 174 0 R >> endobj 174 0 obj << /S /LI /Type /StructElem /K [173 0 R] /P 175 0 R >> endobj 179 0 obj << /S /Span /Type /StructElem /ActualText /K [4] /P 180 0 R /Pg 8 0 R >> endobj 182 0 obj << /S /Span /Type /StructElem /ActualText (2.Selection of Hybrid Cells) /K [5] /P 180 0 R /Pg 8 0 R >> endobj 180 0 obj << /S /LBody /Type /StructElem /K [179 0 R 182 0 R] /P 181 0 R >> endobj 181 0 obj << /S /LI /Type /StructElem /K [180 0 R] /P 175 0 R >> endobj 183 0 obj << /S /Span /Type /StructElem /ActualText /K [6] /P 184 0 R /Pg 8 0 R >> endobj 186 0 obj << /S /Span /Type /StructElem /ActualText (There are three methods for the selection:) /K [7] /P 184 0 R /Pg 8 0 R >> endobj 184 0 obj << /S /LBody /Type /StructElem /K [183 0 R 186 0 R] /P 185 0 R >> endobj 185 0 obj << /S /LI /Type /StructElem /K [184 0 R] /P 175 0 R >> endobj 187 0 obj << /S /Span /Type /StructElem /ActualText /K [8] /P 188 0 R /Pg 8 0 R >> endobj 190 0 obj << /S /Span /Type /StructElem /ActualText (a\) Biochemical method: ) /K [9] /P 188 0 R /Pg 8 0 R >> endobj 191 0 obj << /S /Span /Type /StructElem /ActualText (In this method, biochemical compounds are used to select the ) /K [10] /P 188 0 R /Pg 8 0 R >> endobj 192 0 obj << /S /Span /Type /StructElem /ActualText (fused cells from unfused cells. Drug sensitivity: In this method, one of the protoplasts is ) /K [11] /P 188 0 R /Pg 8 0 R >> endobj 193 0 obj << /S /Span /Type /StructElem /ActualText (resistant to antibiotics and the other protoplast will not be able to grow in its presence. ) /K [12] /P 188 0 R /Pg 8 0 R >> endobj 188 0 obj << /S /LBody /Type /StructElem /K [187 0 R 190 0 R 191 0 R 192 0 R 193 0 R] /P 189 0 R >> endobj 189 0 obj << /S /LI /Type /StructElem /K [188 0 R] /P 175 0 R >> endobj 194 0 obj << /S /Span /Type /StructElem /ActualText /K [13] /P 195 0 R /Pg 8 0 R >> endobj 197 0 obj << /S /Span /Type /StructElem /ActualText (b\) Auxotrophic mutants: ) /K [14] /P 195 0 R /Pg 8 0 R >> endobj 198 0 obj << /S /Span /Type /StructElem /ActualText (Auxotrophs) /K [15] /P 195 0 R /Pg 8 0 R >> endobj 199 0 obj << /S /Span /Type /StructElem /ActualText (are mutants that cannot grow in a minimal medium. ) /K [16] /P 195 0 R /Pg 8 0 R >> endobj 200 0 obj << /S /Span /Type /StructElem /ActualText (The hybrids are able to grow in the minimal medium, whereas the parental cells do not ) /K [17] /P 195 0 R /Pg 8 0 R >> endobj 201 0 obj << /S /Span /Type /StructElem /ActualText (grow, and thus the cells can be selected.) /K [18] /P 195 0 R /Pg 8 0 R >> endobj 195 0 obj << /S /LBody /Type /StructElem /K [194 0 R 197 0 R 198 0 R 199 0 R 200 0 R 201 0 R] /P 196 0 R >> endobj 196 0 obj << /S /LI /Type /StructElem /K [195 0 R] /P 175 0 R >> endobj 202 0 obj << /S /Span /Type /StructElem /ActualText /K [19] /P 203 0 R /Pg 8 0 R >> endobj 205 0 obj << /S /Span /Type /StructElem /ActualText (c\) Visual method: ) /K [20] /P 203 0 R /Pg 8 0 R >> endobj 206 0 obj << /S /Span /Type /StructElem /ActualText (This method is very tedious as it involves selecting the hybrid cells ) /K [21] /P 203 0 R /Pg 8 0 R >> endobj 207 0 obj << /S /Span /Type /StructElem /ActualText (visually and mechanically. In this method, cells that grow on different media are fused to ) /K [22] /P 203 0 R /Pg 8 0 R >> endobj 208 0 obj << /S /Span /Type /StructElem /ActualText (separate them visually after fusion. Another technique is to use a pipette called ) /K [23] /P 203 0 R /Pg 8 0 R >> endobj 209 0 obj << /S /Span /Type /StructElem /ActualText (Drummond pipette to mechanically separate the hybrid cells.) /K [24] /P 203 0 R /Pg 8 0 R >> endobj 203 0 obj << /S /LBody /Type /StructElem /K [202 0 R 205 0 R 206 0 R 207 0 R 208 0 R 209 0 R] /P 204 0 R >> endobj 204 0 obj << /S /LI /Type /StructElem /K [203 0 R] /P 175 0 R >> endobj 210 0 obj << /S /Span /Type /StructElem /ActualText /K [25] /P 211 0 R /Pg 8 0 R >> endobj 213 0 obj << /S /Span /Type /StructElem /ActualText (Cytometric method: ) /K [26] /P 211 0 R /Pg 8 0 R >> endobj 214 0 obj << /S /Span /Type /StructElem /ActualText (Modem techniques such as flow cytometry and fluorescent cells are ) /K [27] /P 211 0 R /Pg 8 0 R >> endobj 215 0 obj << /S /Span /Type /StructElem /ActualText (applied in this method for easy selection of cells.) /K [28] /P 211 0 R /Pg 8 0 R >> endobj 211 0 obj << /S /LBody /Type /StructElem /K [210 0 R 213 0 R 214 0 R 215 0 R] /P 212 0 R >> endobj 212 0 obj << /S /LI /Type /StructElem /K [211 0 R] /P 175 0 R >> endobj 175 0 obj << /S /L /Type /StructElem /K [174 0 R 181 0 R 185 0 R 189 0 R 196 0 R 204 0 R 212 0 R] /P 176 0 R >> endobj 176 0 obj << /S /Textbox /Type /StructElem /K [175 0 R] /P 171 0 R >> endobj 171 0 obj << /S /Slide /Type /StructElem /K [170 0 R 176 0 R] /P 3 0 R >> endobj 217 0 obj << /S /Span /Type /StructElem /K [0] /P 218 0 R /Pg 9 0 R >> endobj 219 0 obj << /S /Span /Type /StructElem /ActualText /K [1] /P 220 0 R /Pg 9 0 R >> endobj 224 0 obj << /S /Span /Type /StructElem /ActualText (3. Identification of Hybrid Plant) /K [2] /P 220 0 R /Pg 9 0 R >> endobj 220 0 obj << /S /LBody /Type /StructElem /K [219 0 R 224 0 R] /P 221 0 R >> endobj 221 0 obj << /S /LI /Type /StructElem /K [220 0 R] /P 222 0 R >> endobj 225 0 obj << /S /Span /Type /StructElem /ActualText /K [3] /P 226 0 R /Pg 9 0 R >> endobj 228 0 obj << /S /Span /Type /StructElem /ActualText (The identification of hybrid plants after development from the hybrid cells requires ) /K [4] /P 226 0 R /Pg 9 0 R >> endobj 229 0 obj << /S /Span /Type /StructElem /ActualText (molecular evidence. Here are some common approaches for the identification of hybrid ) /K [5] /P 226 0 R /Pg 9 0 R >> endobj 230 0 obj << /S /Span /Type /StructElem /ActualText (plants.) /K [6] /P 226 0 R /Pg 9 0 R >> endobj 226 0 obj << /S /LBody /Type /StructElem /K [225 0 R 228 0 R 229 0 R 230 0 R] /P 227 0 R >> endobj 227 0 obj << /S /LI /Type /StructElem /K [226 0 R] /P 222 0 R >> endobj 231 0 obj << /S /Span /Type /StructElem /ActualText /K [7] /P 232 0 R /Pg 9 0 R >> endobj 234 0 obj << /S /Span /Type /StructElem /ActualText (a\) Morphology of hybrid plants: ) /K [8] /P 232 0 R /Pg 9 0 R >> endobj 235 0 obj << /S /Span /Type /StructElem /K [9] /P 232 0 R /Pg 9 0 R >> endobj 236 0 obj << /S /Span /Type /StructElem /ActualText (The morphology of the hybrid plants is usually an ) /K [10] /P 232 0 R /Pg 9 0 R >> endobj 237 0 obj << /S /Span /Type /StructElem /ActualText (intermediate of the two plants and can be identified easily. Hybrids such as ) /K [11] /P 232 0 R /Pg 9 0 R >> endobj 238 0 obj << /S /Span /Type /StructElem /ActualText (pomatoes) /K [12] /P 232 0 R /Pg 9 0 R >> endobj 239 0 obj << /S /Span /Type /StructElem /ActualText (and ) /K [13] /P 232 0 R /Pg 9 0 R >> endobj 240 0 obj << /S /Span /Type /StructElem /ActualText (topatoes) /K [14] /P 232 0 R /Pg 9 0 R >> endobj 241 0 obj << /S /Span /Type /StructElem /ActualText (are fusions of tomatoes and potatoes and can be easily distinguished from ) /K [15] /P 232 0 R /Pg 9 0 R >> endobj 242 0 obj << /S /Span /Type /StructElem /ActualText (the mother plant.) /K [16] /P 232 0 R /Pg 9 0 R >> endobj 232 0 obj << /S /LBody /Type /StructElem /K [231 0 R 234 0 R 235 0 R 236 0 R 237 0 R 238 0 R 239 0 R 240 0 R 241 0 R 242 0 R] /P 233 0 R >> endobj 233 0 obj << /S /LI /Type /StructElem /K [232 0 R] /P 222 0 R >> endobj 243 0 obj << /S /Span /Type /StructElem /ActualText /K [17] /P 244 0 R /Pg 9 0 R >> endobj 246 0 obj << /S /Span /Type /StructElem /ActualText (b\) Isoenzyme analysis of hybrid plants: ) /K [18] /P 244 0 R /Pg 9 0 R >> endobj 247 0 obj << /S /Span /Type /StructElem /ActualText (Isoenzymes are forms of the same enzymes that ) /K [19] /P 244 0 R /Pg 9 0 R >> endobj 248 0 obj << /S /Span /Type /StructElem /ActualText (catalyze different reactions. The hybrid plant consists of isoenzymes from one or both ) /K [20] /P 244 0 R /Pg 9 0 R >> endobj 249 0 obj << /S /Span /Type /StructElem /ActualText (the parents. These isoenzymes can be ) /K [21] /P 244 0 R /Pg 9 0 R >> endobj 250 0 obj << /S /Span /Type /StructElem /ActualText (analysed) /K [22] /P 244 0 R /Pg 9 0 R >> endobj 251 0 obj << /S /Span /Type /StructElem /ActualText (electrophoretically) /K [23] /P 244 0 R /Pg 9 0 R >> endobj 252 0 obj << /S /Span /Type /StructElem /ActualText (to verify hybridity.) /K [24] /P 244 0 R /Pg 9 0 R >> endobj 244 0 obj << /S /LBody /Type /StructElem /K [243 0 R 246 0 R 247 0 R 248 0 R 249 0 R 250 0 R 251 0 R 252 0 R] /P 245 0 R >> endobj 245 0 obj << /S /LI /Type /StructElem /K [244 0 R] /P 222 0 R >> endobj 253 0 obj << /S /Span /Type /StructElem /ActualText /K [25] /P 254 0 R /Pg 9 0 R >> endobj 256 0 obj << /S /Span /Type /StructElem /ActualText (c\) Symmetric and asymmetric hybrids: ) /K [26] /P 254 0 R /Pg 9 0 R >> endobj 257 0 obj << /S /Span /Type /StructElem /ActualText (When hybrid plants contain the chromosome ) /K [27] /P 254 0 R /Pg 9 0 R >> endobj 258 0 obj << /S /Span /Type /StructElem /ActualText (numbers the same as their parents, they are called symmetric hybrids and are sterile in ) /K [28] /P 254 0 R /Pg 9 0 R >> endobj 259 0 obj << /S /Span /Type /StructElem /ActualText (nature. Asymmetric hybrids are however abnormal and do not have a normal ) /K [29] /P 254 0 R /Pg 9 0 R >> endobj 260 0 obj << /S /Span /Type /StructElem /ActualText (chromosome number or ploidy.) /K [30] /P 254 0 R /Pg 9 0 R >> endobj 254 0 obj << /S /LBody /Type /StructElem /K [253 0 R 256 0 R 257 0 R 258 0 R 259 0 R 260 0 R] /P 255 0 R >> endobj 255 0 obj << /S /LI /Type /StructElem /K [254 0 R] /P 222 0 R >> endobj 222 0 obj << /S /L /Type /StructElem /K [221 0 R 227 0 R 233 0 R 245 0 R 255 0 R] /P 223 0 R >> endobj 223 0 obj << /S /Textbox /Type /StructElem /K [222 0 R] /P 218 0 R >> endobj 218 0 obj << /S /Slide /Type /StructElem /K [217 0 R 223 0 R] /P 3 0 R >> endobj 60 0 obj << /Nums [0 [22 0 R 24 0 R 27 0 R 28 0 R 29 0 R 30 0 R 31 0 R 32 0 R 34 0 R 35 0 R 38 0 R 39 0 R 40 0 R 41 0 R 42 0 R 44 0 R 45 0 R 47 0 R 49 0 R 50 0 R 53 0 R 54 0 R 56 0 R 58 0 R 59 0 R] 1 [75 0 R 77 0 R 82 0 R 83 0 R 86 0 R 87 0 R 88 0 R 91 0 R 92 0 R 95 0 R 96 0 R 97 0 R 98 0 R] 2 [103 0 R 105 0 R 110 0 R 111 0 R 112 0 R 115 0 R 116 0 R 119 0 R 120 0 R 121 0 R 122 0 R 125 0 R 126 0 R 127 0 R 128 0 R 129 0 R 132 0 R 133 0 R 134 0 R 135 0 R 136 0 R 137 0 R 140 0 R 141 0 R 144 0 R 145 0 R 146 0 R 147 0 R 148 0 R 149 0 R 150 0 R 151 0 R 154 0 R 155 0 R 156 0 R 157 0 R 158 0 R 159 0 R 160 0 R 163 0 R 164 0 R 165 0 R 166 0 R 167 0 R 168 0 R] 3 [170 0 R 172 0 R 177 0 R 178 0 R 179 0 R 182 0 R 183 0 R 186 0 R 187 0 R 190 0 R 191 0 R 192 0 R 193 0 R 194 0 R 197 0 R 198 0 R 199 0 R 200 0 R 201 0 R 202 0 R 205 0 R 206 0 R 207 0 R 208 0 R 209 0 R 210 0 R 213 0 R 214 0 R 215 0 R] 4 [217 0 R 219 0 R 224 0 R 225 0 R 228 0 R 229 0 R 230 0 R 231 0 R 234 0 R 235 0 R 236 0 R 237 0 R 238 0 R 239 0 R 240 0 R 241 0 R 242 0 R 243 0 R 246 0 R 247 0 R 248 0 R 249 0 R 250 0 R 251 0 R 252 0 R 253 0 R 256 0 R 257 0 R 258 0 R 259 0 R 260 0 R]] >> endobj 4 0 obj << /Footnote /Note /Endnote /Note /Textbox /Sect /Header /Sect /Footer /Sect /InlineShape /Sect /Annotation /Sect /Artifact /Sect /Workbook /Document /Worksheet 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